화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.23, No.3, 471-477, May, 1999
Export Citation
유화동결 건조방법에 의한 튜브형태의 다공성 PLGA 지지체의 제조
Fabrication of Tubular Porous PLGA Scaffold by Emulsion Freeze-drying Method
강길선, 전주형1, 조진철1, 이해방1, †
  • 전북대학교 고분자공학과
  • 1한국화학연구소 생체고분자실
Gilson Khang, Ju Kyeong Jeon1, Jin Cheol Cho1, and Hai Bang Lee1, †
  • Biomaterials Laboratory, Korea Research Insitute of Chemical Technology, P. O. Box 107, Yusung, Taejon 305-343, Korea
  • 1Biomaterials Laboratory, Korea Research Institute of Chemical Technology, P. O. Box 107, Yusung, Taejon 305-343, Korea
E-mail:
초록
인체조직 중에서 비교적 높은 빈도수를 찾아 볼 수 있는 장기의 형태인 튜브형태의 다공성 poly(L-lactide-co-glycolide) (75 : 25 by mole ratio of lactide to glycolide, PLGA) 지지체를 세포조직공학에 응용하기 위하여 유화동결 건조법으로 제조하였고 이들의 물성을 수은다공도계, 전자현미경 및 이미지분석으로 특성결정하였다. PLGA 농도 및 물의 양을 조절하여 제조된 PLGA 지지체의 기공도는 90% 이상이었으며 평균 기공크기 범위는 10-30 ㎛ 그리고 가장 큰 것으로는 100 ㎛ 이상의 것도 관찰되었다. 또한 비기공 면적은 27-31 (m+/g)에 걸쳐 있었으며 기공과 기공사이의 연결은 세포가 성장하기에 적합한 연결된 구조로 확인되었다. 평균 기공크기와 여타이 물성은 PLGA 용액의 농도 및 유화를 생성할 때 첨가되는 물의 양에 의하여 비교적 용이하게 조절되었다. 본 연구의 이러한 결과는 기공의 구조 생성에 있어서 유화 제조시 유화의 안정화보다는 불안정화가 우세하게 기여함을 알 수 있었다.
A novel emulsion freeze-drying method for fabrication of tubular porous poly(L-lactide-co-glycolide) (75:25 by mole ratio of lactide to glycolide, PLGA) scaffold has been proposed for the application to tissue engineering. Tubular PLGA scaffolds obtained were characterized by mercury porosimetry, scanning electron microscopy and image analysis. PLGA scaffolds with porosity above 90%, medium pore size ranging from 10 to 30 ㎛ with larger pore diameters greater than 100 ㎛, and specific pore area in the range of 27 to 31 (㎡/g) were manufactured by varying processing parameters as PLGA concentration and water volume fraction. It was observed that PLGA scaffolds were highly porous with good interconnections between pores for allowing cell adhesion and growth. It was suggested that the mechanism of the formation pore may be related to emulsion unstability rather than emulsion stability. It might be concluded that this novel scaffolds may be applicable for tubular organ structures to facilitate tissue regeneration or repair in tissue engineering.
Keywords:tubular porous PLGA scaffolds;emulsion freeze-drying;tissue engineering;pore structure
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