A simple electrochemical method for the highly Sensitive determination of hyperin was established based on poly(diallyldimethylammonium chloride)-functionalized graphene modified electrode (PDDA-Gr/GCE). The nanocomposite was characterized by X-ray diffraction (XRD), ultraviolet/visible spectra (UV-vis), Fourier Transform infrared spectroscopy (FT-IR) and transmission electron microscopy (TEM). The electrochemical characteristics of hyperin were studied in detail via cyclic voltammetry (CV), chronocoulometry (CC). Dynamics parameters of electrode process were also calculated to discuss the reaction mechanism. Under the optimized conditions, a lower detection limit of 5 x 10(-9) mol L-1 (S/N = 3) and a wide linear detection range from 7 x 10(-9) to 7 x 10(-7) mol L-1 were achieved by the differential-pulse voltammetry (DPV). Additionally, this simple, sensitive sensor was proved to be suitable for the determination of hyperin in Chinese herb Hypericum Perforatum with satisfactory results. (C) 2016 Elsevier B.V. All rights reserved.