Exergy analysis gives the presentation of a system relative to its best performance. In addition, the exergy destructed can react with its surrounding and harm environment processes. This study investigated the effect of biodiesel fuel blended with diesel fuel (i.e. 0%, 20%, and 50% blending of biodiesel fuel with conventional diesel fuel) on various exergy terms in an HCCI engine. To model the energy balance a 3-D CFD code was utilized. Using energy and combustion analyses results, the researchers calculated various exergy terms by developing a FORTRAN based code. To ensure the integrity of modeling, the results of the in-cylinder pressure and heat release rate were compared with the experimental results for pure diesel fuel. This comparison indicated a good agreement between the two. With crank position at three fuel compositions, different rates of exergy and cumulative exergy terms were identified and calculated separately. With the increase in the biodiesel volume percentage from 0% to 20% and 50%, exergy efficiency increased by 4.9% and 5.7%. Also, the cumulative heat loss exergy decreased by 4.4% and 9.7%, respectively. (C) 2016 Elsevier Ltd. All rights reserved.