The new ion exchange resin was synthesized from chloromethylated styrene-1,4-divinylbenzene with crown macrocyclic ligand by substitution reaction. The resin was prepared and the effect of pH, time, dielectric constant of solvent and crosslinked of the matrix on the adsorption for UO2(2+), Zn(2+) and Ce(3+) was investigated. The metal ion was not adsorbed on the resins pH range below 3 and above pH 4 fast adsorption behavior was showed. The resin selectivity determined in ethanol matrix was in increasing order UO2(2+)>Zn(2+)>Ce(3+). In addition, these metal ions was separated on the column packed with 1% crosslinked resin as an eluent.