In this study, bioflocculant produced by Pseudomonas veronii L918 was applied to treat ash-flushing wastewater. The strain L918 could convert the hydrolyzate of peanut hull into bioflocculant, which can effectively reduce the production cost of bioflocculant. The yield of 3.39 g/L bioflocculant MBF-L918 was achieved when 300 mL/L peanut hull hydrolyzate was used as carbon source. The bioflocculant MBF-L918 contains 77.14% polysaccharides and 4.84% proteins, and the molecular weight (MW) of MBF-L918 is 24.77 kDa. Furthermore, MBF-L918 showed good flocculating efficiency of 92.51% to ash-flushing wastewater when 2.83 mg/L MBF-L918 was added, and thus achieved the recycling of ash-flushing wastewater. This study reported for the first time that the bioflocculant was produced using peanut hull hydrolyzate and effectively applied in the treatment of coal ash-flushing wastewater. (C) 2016 Elsevier Ltd. All rights reserved.