This paper analyses concepts to facilitate a demand oriented biogas supply at an agricultural biogas plant of a capacity of 500 kWh el, operated with the co-digestion of maize, grass, rye silage and chicken manure. In contrast to previous studies, environmental impacts of flexible and the traditional baseload operation are compared. Life Cycle Assessment (LCA) was performed to detect the environmental impacts of: (i) variety of feedstock co-digestion scenarios by substitution of maize and (ii) loading rate scenarios with a focus on flexible feedstock utilization. Demand-driven biogas production is critical for an overall balanced power supply to the electrical grid. It results in lower amounts of emissions; feedstock loading rate scenarios resulted in 48%, 20%, 11% lower global warming (GWP), acidification (AP) and eutrophication potentials, and a 16% higher cumulative energy demand. Substitution of maize with biogenic-waste regarding to feedstock substitution scenarios could create 10% lower GWP and AP. (C) 2016 Elsevier Ltd. All rights reserved.