화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.24, No.6, 869-876, November, 2000
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물리화학적 처리된 PLGA 표면의 섬유아세포와의 상호작용
Interaction of Fibroblast Cell onto Physicochemically Treated PLGA Surfaces
강길선, 이상진1, 전주형2, 이진호1, 이해방2, †
  • 전북대학교 고분자공학고
  • 1한남대학교 고분자공학과
  • 2한국화학연구소 생체고분자팀
Gilson Khang, Sang Jin Lee1, Ju Hyeong Jeon2, Jin Ho Lee1, and Hai Bang Lee2, †
  • Department of Polymer Science and Technology, Chonbuk National University, 664-14, Dukjin Dong 1Ga, Dukjin Ku, Chonju 561-756, Korea
  • 1Department of Polymer Science and Engineering, Hanyang University, 133 Ojeong Dong, Daedeog Ku, Taejon 306-741, Korea
  • 2Biomaterials Laboratory, Korea Research Institute of Chemical Technology, P. O. Box 107, Yusung, Taejon 305-343, Korea
E-mail:
초록
소수성 표면성질을 나타내고 있는 poly(L-lactide-co-glycolide) (75:25 by mole ratio of lactide to glycolide, PLGA) 표면의 세포적합성을 증가시키기 위하여 물리화학적 처리방법으로 표면개질 하였다. 물리적 방법으로는 코로나 방전 및 플라즈마 처리가, 화학적인 방법으로는 70% 염소산, 50% 황산 및 0.5 N 가성소다 수용액으로 처리하였다. 처리된 PLGA 표면의 물접촉각은 73 ℃에서 50~60°로 감소하였고 electron spectroscopy for chemical analysis의 분석결과, 알킬탄소는 감소되는 반면, 산소를 포함하는 탄소 관능기들이 상대적으로 증가함을 보여 친수화의 주원인으로 사료되었다. 섬유아세포의 배양 결과 control에 비하여 처리된 PLGA상에 점착 및 성장 거동이 우월하게 관측되어 본 처리 방법이 세포적합성을 증가시켰다고 사료된다. 결론적으로 PLGA 표면의 적심성에 있어서 친수화는 세포의 점착 및 성장에 중요한 역할을 하는 것으로 확인되었다.
In order to improve the cell-compatability of poly(L-lactide-co-glycolide)(75:25 by mole ratio of lactide to glycolide, PLGA) surfaces, the physicochemical treatments have been demonstrated. Chemical treatments were 70% perchloric acid, 50% sulfuric acid and 0.5 N sodium hydroxide solution and physical methods were corona and plasma treatment. The water contact angle of surface treated PLGA decreased from 73° to 50∼60°, i.e., increased hydrophilicity, due to the introduction of oxygen-containing functional group onto PLGA backbone by the measurement of an electron spectroscopy for chemical analysis. It could be observed that the adhesion and growth of fibroblast cell on physicochemically treated PLGA surface, especially perchloric acid treated PLGA surface, were more active than on the control. In conclusion, it seems that surface wettability as hydrophilicity of PLGA plays an important role in cell adhesion, spreading and growth.
Keywords:PLGA surface;physicochemical treatment;wettability;fibroblast cell adhesion and growth;perchloric acid treatment
  1. Langer R, Vacanti JP, Science, 260, 920 (1993) 
  2. Khang G, Lee JH, "The Properties of Biomaterials," in "Tissue Engineering: Concepts and Applications," J.J. Yoo and I. Lee eds., Korea Med. Pub. Co., Seoul, 1998 (1998)
  3. Vacanti JP, Morse MA, Saltzman WM, Domb AJ, Perez-Atayde A, Langer R, J. Pediatr. Surg., 23, 3 (1998) 
  4. Khang G, Lee HB, Chem. World, 37(3), 46 (1997)
  5. Khang G, Lee HB, J. Biomed. Eng. Res., 20(1), 1 (1999)
  6. Khang G, Jo I, Lee JH, Lee I, Lee HB, Polym. Sci. Technol., 10(5), 640 (1999)
  7. Khang G, Lee HB, Bioindustry, 22, 32 (1999)
  8. Khang G, Lee JH, Lee HB, Polym. Sci. Technol., 10(6), 732 (1999)
  9. Lee I, Khang G, Lee HB, Polym. Sci. Technol., 10(6), 782 (1999)
  10. Holland SJ, Tighe BJ, Gould PL, J. Control. Release, 4, 155 (1986) 
  11. Khang G, Jeon JH, Lee JW, Cho SC, Lee HB, Bio-Med. Mater. Eng., 7(6), 357 (1997)
  12. Khang G, Lee SJ, Lee JH, Kim YS, Lee HB, Bio-Med. Mater. Eng., 9(3), 179 (1999)
  13. Williams DF, Mort E, J. Bioeng., 1, 231 (1997)
  14. Bostaman O, J. Bone Joint Surg., 73A(1), 148 (1991)
  15. Singhal JP, Singh H, Ray AR, Rev. Macromol. Chem. Phys., C28, 475 (1988)
  16. Hollinger OJ, Schmitz JP, J. Oral Maxillofac. Surg., 45, 594 (1987)
  17. Khang G, Cho JC, Lee JW, Rhee JM, Lee HB, Bio-Med. Mater. Eng., 9(1), 49 (1999)
  18. Cho JC, Khang G, Rhee JM, Kim YS, Lee JS, Lee HB, Korea Polym. J., 7(2), 79 (1999)
  19. Lee HB, Khang G, Cho JC, Rhee JM, Lee JS, Polym. Prepr., 40, 288 (1999)
  20. Khang G, Jeon JH, Cho JC, Lee HB, Polym.(Korea), 23(3), 471 (1999)
  21. Khang G, Jeon JH, Cho JC, Rhee JM, Lee HB, Polym.(Korea), 23(6), 861 (1999)
  22. Mikos AG, Sarakinos G, Leite SM, Vacanti JP, Langer R, Biomaterials, 14(5), 323 (1993) 
  23. Lee JH, Khang G, Park KH, Lee HB, Andrade JD, J. Biomed. Eng. Res., 10, 43 (1989)
  24. Lee JH, Khang G, Park KH, Lee HB, Andrade JD, J. Biomed. Eng. Res., 10, 195 (1989)
  25. Khang G, Lee JW, Jeon JH, Lee JH, Lee HB, Biomater. Res., 1, 1 (1997)
  26. Lee JH, Lee SJ, Khang G, Lee HB, J. Biomater. Sci.-Polym. Ed., 10, 283 (1999)
  27. Khang G, Lee SJ, Lee JH, Lee HB, Korea Polym. J., 7(2), 102 (1999)
  28. Khang G, Kang YH, Park JB, Lee HB, Bio-Med. Mater. Eng., 6, 335 (1996)
  29. Khang G, Lee HB, Park JB, Bio-Med. Mater. Eng., 5, 245 (1996)
  30. Khang G, Jeong BJ, Lee HB, Park JB, Bio-Med. Mater. Eng., 5, 259 (1995)
  31. Lee JH, Khang G, Lee JW, Lee HB, J. Colloid Interface Sci., 205(2), 323 (1998) 
  32. Iwasaki Y, Ishihara K, Nakabayashi N, Khang G, Jeon JH, Lee JW, Lee HB, J. Biomater. Sci.-Polym. Ed., 9, 801 (1998)
  33. Lee JH, Khang G, Lee JW, Lee HB, Makromol. Chem. Makromol. Symp., 118, 571 (1997)
  34. Lee JH, Lee JW, Khang G, Lee HB, Biomaterials, 18, 351 (1997) 
  35. Khang G, Jeon JH, Lee JW, Lee HB, Bio-Med. Mater. Eng., 8(5-6), 299 (1998)
  36. van Wachem PB, Beugeling T, Feijen J, Bantjes A, Detmers JP, van Aken WG, Biomaterials, 6, 403 (1985) 
  37. van Wachem PB, Hogt AH, Beugeling T, Biomaterials, 8, 323 (1987) 
  38. Steele JG, Dalton BA, Johnson G, Underwood PA, Biomaterials, 16, 1057 (1995) 
  39. Underwood PA, Bennett FA, J. Cell. Sci., 93, 641 (1989)
  40. Steele JG, Johnson G, Underwood PA, J. Biomed. Mater. Res., 26, 861 (1992) 
  41. Hass R, Culp LA, J. Cell. Physiol., 113, 289 (1982) 
  42. Knox P, J. Cell. Sci., 71, 51 (1984)
  43. Steele JG, Johnson G, Griesser G, J. Biomater. Sci.-Polym. Ed., 6, 511 (1994)
  44. Underwood PA, Bennett FA, Exp. Cell. Res., 205, 311 (1993)