The aim of this paper was to present the energy flows in the life cycle of biogas utilization systems (cogeneration and transportation), as well as their mutual relations, starting from providing the feedstock for digestion through to end-of-life management of biogas system as fertilizer on agricultural land. This study was carried out through the energy analysis of two scenarios (biogas in cogeneration and biogas in transportation) using performance of Mirotin biogas plant (1 MW) in Serbia. Results obtained in this study have shown that the analyzed scenario (biogas in cogeneration and biogas in transportation) have positive energy balances (52,114 and 53,585 GJ) and these scenarios are sustainable from energetic point of view.