The current experimental work focusses on influence of Alumina (Al2O3) nanoparticle on various injection strategies. Experiments were conducted with three different injection timings (IT) namely, original timing (ORG IT) of 23 deg bTDC, advanced timing (ADV IT) of 27 deg bTDC and retarded timing (RET IT) of 19 deg bTDC. The base fuel used is a blend of biodiesel (20%), diesel (70%), and ethanol (10%) (known as BDE). Alumina nanoparticles were synthesized, characterized by SEM and XRD analysis and blended with BDE blend at a fraction of 25 ppm using ultrasonicator. The effect of 25 ppm Al2O3 in BDE blend at ORG IT, ADV IT and RET IT were experimented in a single cylinder diesel engine and the following results were obtained. Al2O3 addition at ADV IT resulted in higher peak pressure and heat release rate occurring nearer to TDC, higher hydrocarbon (HC), higher carbon monoxide (CO), lower carbon dioxide (CO2), higher nitrogen oxides (NOx), higher exhaust gas oxygen (EGO), higher combustion duration (CD) and lower ignition delay (ID). Whereas, Al2O3 addition in RET IT causes lower cylinder pressure and heat release away from TDC, followed by simultaneous reductions of HC, CO, NOx and smoke opacity. In addition, higher levels of EGO and ID along with lowered brake specific fuel consumption (BSFC) and CD were observed with Al2O3 addition in RET IT. Overall, the influence of 25 ppm Al2O3 in RET IT of 19 deg bTDC resulted in better engine performance, combustion and emission characteristics. (C) 2016 Elsevier Ltd. All rights reserved.