The effects of the composition of the microporous layer (MPL) on the through-plane permeability of the gas diffusion layers (GDLs) used in polymer electrolyte fuel cells (PEFCs) have been thoroughly experimentally investigated in this paper. For a given PTFE loading in the MPL, the GDL permeability was found to decrease with increasing carbon loading and this is due to the increase in the thickness of the MPL. For all the investigated carbon loadings of the MPL, the permeability values of the GDLs were found to have common trends for the PTFE loadings ranging from 10 to 50% (by weight): the GDL permeability increases when the PTFE loading in the MPL is increased from 20 to 50%; the GDL permeability decreases when the PTFE loading in the MPL is increased from 10 to 20%; and the GDL permeability is a minimum at 20% PTFE loading present in the MPL. On the other hand, the permeability of the GDL was found to depend on the carbon loading of the MPL in the PTFE range 0-10%. The effects of the MPL composition on the MPL permeability were found to be similar to those on the GDL permeability. However, the permeability values of the MPLs of the same composition, which were supposed to be ideally the same, were found to significantly vary. This was attributed to the MPL penetration into the body of the carbon substrates. (C) 2016 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.