화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.41, No.1, 143-150, January, 2017
DOI10.7317/pk.2017.41.1.143  Export Citation
감마선 이용 AEMA 도입 PCL 나노섬유의 제조 및 특성분석
Preparation and Characterization of AEMA Grafted PCL Nanofibers Using Gamma-ray Irradiation
서다은, 정성린1, 박종석1, 권희정1, 안성준1, 이병헌1, 임돈순1, 신흥수, 임윤묵1, †
  • 한양대학교 생명공학과
  • 1한국원자력연구원 첨단방사선연구소 공업환경연구부
Da-Eun Seo, Sung In Jeong1, Jong-Seok Park1, Hui-Jeong Gwon1, Sung-Jun An1, Byoung Hun Lee1, Don-Sun Im1, Heungsoo Shin, and Youn-Mook Lim1, †
  • Department of Bioengineering, Division of Applied Chemical and Bio Engineering, Hanyang University, Seoul 04763, Korea
  • 1Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 1266 Sinjeong-dong, Jeongeup-si, Jeollabuk-do 56212, Korea
E-mail:
초록
폴리카프로락톤(polycaprolactone, PCL)은 생체적합성이 우수하고 시간이 지나면 체내에서 생분해되어 조직공학용 생체재료로 많이 사용되고 있다. 하지만, 소수성 표면과 낮은 세포 부착성을 나타내는 단점이 있다. 따라서 본 연구에서는 PCL 필름 및 전기방사를 통한 나노섬유 지지체를 제조하고 감마선을 이용하여 친수성과 양전하의 아민기를 갖는 2-아미노에틸메타크릴레이트(2-aminoethylmethacrylate, AEMA)로 표면을 개질하였다. 감마선을 이용하여 표면 개질된 PCL 필름과 나노섬유의 분석 결과, ATR-FTIR을 통해 감마선 선량과 반응 표면적이 증가할수록 표면의 아민기가 증가함을 확인하였다. 아민기의 증가로 인해 친수성이 증가했음을 확인하였고 단백질 흡착량이 정전기적 인력으로 인해 증가함을 확인하였다. 이를 통해 AEMA가 도입된 PCL 필름보다 나노섬유 지지체의 경우 세포적합성이 향상된 조직공학용 지지체로서의 응용 가능성이 매우 높을 것으로 기대된다.
Polycaprolactone (PCL) has been applied to tissue engineering because it has excellent biocompatibility and biodegradable properties. However, there is a limit to hydrophobic surface and low cell adhesion, therefore the PCL requires surface modification for increasing hydrophilicity and cell adhesion capacity. In this study, PCL films and nanofibers were manufactured by solvent casting and electrospinning, respectively. They were modified by 2-aminoethylmethacrylate (AEMA) that had hydrophilicity and amine groups of positive charge using gamma-irradiation. The surface properties of AEMA-grafted PCL films and nanofibers were confirmed by ATR-FTIR, and amine groups were increased by surface area and gamma-irradiation dose. A significantly higher amount of negative-charged proteins adhered to hydrophilic and cationic surface of AEMA-grafted PCL nanofibers than that of AEMA-grafted PCL films, because of increased amine groups and electrostatic interaction. The results show that AEMA-grafted PCL nanofibers are possible to apply tissue engineering scaffolds.
Keywords:polycaprolactone;2-aminoethylmethacrylate;surface modification;positive charge;gamma irradiation
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