Development of a continuous L-lysine bioconversion system for cadaverine production

Jung-Ho Kim; Hyung-Min Seo; Ganesan Sathiyanarayanan; Shashi Kant Bhatia; Hun-Suk Song; Junyoung Kim; Jong-Min Jeon; Jeong-Jun Yoon; Yun-Gon Kim; Kyungmoon Park; Yung-Hun Yang

Journal of Industrial and Engineering Chemistry, Vol.46, 44-48, February, 2017

DOI10.1016/j.jiec.2016.09.038 Export Citation

Development of a continuous L-lysine bioconversion system for cadaverine production

Jung-Ho Kim¹, Hyung-Min Seo¹, Ganesan Sathiyanarayanan¹, Shashi Kant Bhatia¹, Hun-Suk Song¹, Junyoung Kim¹, Jong-Min Jeon¹, Jeong-Jun Yoon², Yun-Gon Kim³, Kyungmoon Park⁴, and Yung-Hun Yang^{1, 5, †}

¹Department of Biological Engineering, College of Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea
²IT Convergence Materials R&BD Group, Chungcheong Regional Division, Korea Institute of Industrial Technology (KITECH), 35-3 Hongchon-ri, Ipjang-myun, Seobuk-gu, Chonan-si, Chungnam 330-825, Republic of Korea
³Chemical Engineering, Soongsil University, 511 Sangdo-dong, Seoul 156-743, Republic of Korea
⁴Department of Biological and Chemical Engineering, Hongik University, Sejong Ro 2639, Jochiwon, Sejong City 339-701, Republic of Korea
⁵Institute for Ubiquitous Information Technology and Applications (CBRU), Konkuk University, Seoul 143-701, Republic of Korea

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Cadaverine, a five carbon diamine (1,5-diaminopentane), plays a role as a building block of polyamides and it can be made by fermentation or direct bioconversion. To improve its production by increasing reusability of immobilized enzyme and avoid separation of enzyme in bioconversion, a continuous L-lysine bioconversion process for cadaverine production has been developed. Various divalent cations, alginate concentrations, cell density with alginate and flow rate of feed were examined to maximize the lysine decarboxylase activity of the whole-cell immobilized beads. Under the selected conditions,123 h of continuous cadaverine production has been performed and 5.5 L of 819 mM cadaverine were produced with 14 mL reactor resulting in 466.5 g of cadaverine. Cadaverine production was possible with small volume reactor maintaining relatively high concentration of substrate.

Keywords:Cadaverine;Bioconversion;Immobilization;Continuous production;Packed-bed reactor

[References]

Jang YS, Kim B, Shin JH, Choi YJ, Choi S, Song CW, Lee J, Park HG, Lee SY, Biotechnol. Bioeng., 109(10), 2437 (2012)
Kamm B, Kamm M, Chem. Ing. Tech., 79(5), 592 (2007)
Menrad K, Klein A, Kurka S, Biofuels Bioprod. Bioref. Biofpr, 3, 384 (2009)
Becker J, Lange A, Fabarius J, Wittmann C, Curr. Opin. Biotechnol., 36, 168 (2015)
Zeng AP, Biebl H, Adv. Biochem. Eng. Biotechnol., 74, 239 (2002)
Shin JH, Kim HU, Kim DI, Lee SY, Biotechnol. Adv., 31, 925 (2013)
Kind S, Neubauer S, Becker J, Yamamoto M, Volkert M, von Abendroth G, Zelder O, Wittmann C, Metab. Eng., 25, 113 (2014)
Ikeda N, Miyamoto M, Adachi N, Nakano M, Tanaka T, Kondo A, AMB Express, 3, 67 (2013)
Tateno T, Okada Y, Tsuchidate T, Tanaka T, Fukuda H, Kondo A, Appl. Microbiol. Biotechnol., 82(1), 115 (2009)
Qian ZG, Xia XX, Lee SY, Biotechnol. Bioeng., 108(1), 93 (2011)
Mimitsuka T, Sawai H, Hatsu M, Yamada K, Biosci. Biotechnol. Biochem., 71, 2130 (2007)
Lessmeier L, Pfeifenschneider J, Carnicer M, Heux S, Portais JC, Wendisch VF, Appl. Microbiol. Biotechnol., 99(23), 10163 (2015)
Buschke N, Becker J, Schafer R, Kiefer P, Biedendieck R, Wittmann C, Biotechnol. J., 8, 557 (2013)
Oh YH, Kang KH, Kwon MJ, Choi JW, Joo JC, Lee SH, Yang YH, Song BK, Kim IK, Yoon KH, Park K, Park SJ, J. Ind. Microbiol. Biotechnol., 42, 1481 (2015)
Kim HJ, Kim YH, Shin JH, Bhatia SK, Sathiyanarayanan G, Seo HM, Choi KY, Yang YH, Park K, J. Microbiol. Biotechnol., 25, 1108 (2015)
Ma WC, Cao WJ, Zhang H, Chen KQ, Li Y, Ouyang PK, Biotechnol. Lett., 37(4), 799 (2015)
Bhatia SK, Kim YH, Kim HJ, Seo HM, Kim JH, Song HS, Sathiyanar-ayanan G, Park SH, Park K, Yang YH, Bioprocess. Biosyst. Eng., 38, 2315 (2015)
Friedl A, Qureshi N, Maddox IS, Biotechnol. Bioeng., 38, 518 (1991)
Nakasaki K, Murai T, Akiyama T, Biotechnol. Bioeng., 33, 1317 (1989)
Duarte JC, Rodrigues JA, Moran PJ, Valenca GP, Nunhez JR, AMB Express, 3, 31 (2013)
Najafpour G, Younesi H, Ismail KSK, Bioresour. Technol., 92(3), 251 (2004)
Barros MR, Cabral JM, Novais JM, Biotechnol. Bioeng., 29, 1097 (1987)
Park MC, Lim JS, Kim JC, Park SW, Kim SW, Biotechnol. Lett., 27, 127 (2005)
Lorenzoni ASG, Aydos LF, Klein MP, Ayub MAZ, Rodrigues RC, Hertz PF, J. Mol. Catal. B-Enzym., 111, 51 (2015)
Agulhon P, Markova V, Robitzer M, Quignard F, Mineva T, Biomacromolecules, 13(6), 1899 (2012)
Morch YA, Donati I, Strand BL, Skjak-Braek G, Biomacromolecules, 7(5), 1471 (2006)
Mahajan R, Gupta VK, Sharma J, Indian J. Pharm. Sci., 72, 223 (2010)
Bajpai SK, Sharma S, React. Funct. Polym., 59(2), 129 (2004)
Kim YH, Kim HJ, Shin JH, Bhatia SK, Seo HM, Kim YG, Lee YK, Yang YH, Park K, J. Mol. Catal. B-Enzym., 115, 151 (2015)

[Referenced By]

[1] Jang YS, Kim B, Shin JH, Choi YJ, Choi S, Song CW, Lee J, Park HG, Lee SY, Biotechnol. Bioeng., 109(10), 2437 (2012)

[2] Kamm B, Kamm M, Chem. Ing. Tech., 79(5), 592 (2007)

[3] Menrad K, Klein A, Kurka S, Biofuels Bioprod. Bioref. Biofpr, 3, 384 (2009)

[4] Becker J, Lange A, Fabarius J, Wittmann C, Curr. Opin. Biotechnol., 36, 168 (2015)

[5] Zeng AP, Biebl H, Adv. Biochem. Eng. Biotechnol., 74, 239 (2002)

[6] Shin JH, Kim HU, Kim DI, Lee SY, Biotechnol. Adv., 31, 925 (2013)

[7] Kind S, Neubauer S, Becker J, Yamamoto M, Volkert M, von Abendroth G, Zelder O, Wittmann C, Metab. Eng., 25, 113 (2014)

[8] Ikeda N, Miyamoto M, Adachi N, Nakano M, Tanaka T, Kondo A, AMB Express, 3, 67 (2013)

[9] Tateno T, Okada Y, Tsuchidate T, Tanaka T, Fukuda H, Kondo A, Appl. Microbiol. Biotechnol., 82(1), 115 (2009)

[10] Qian ZG, Xia XX, Lee SY, Biotechnol. Bioeng., 108(1), 93 (2011)

[11] Mimitsuka T, Sawai H, Hatsu M, Yamada K, Biosci. Biotechnol. Biochem., 71, 2130 (2007)

[12] Lessmeier L, Pfeifenschneider J, Carnicer M, Heux S, Portais JC, Wendisch VF, Appl. Microbiol. Biotechnol., 99(23), 10163 (2015)

[13] Buschke N, Becker J, Schafer R, Kiefer P, Biedendieck R, Wittmann C, Biotechnol. J., 8, 557 (2013)

[14] Oh YH, Kang KH, Kwon MJ, Choi JW, Joo JC, Lee SH, Yang YH, Song BK, Kim IK, Yoon KH, Park K, Park SJ, J. Ind. Microbiol. Biotechnol., 42, 1481 (2015)

[15] Kim HJ, Kim YH, Shin JH, Bhatia SK, Sathiyanarayanan G, Seo HM, Choi KY, Yang YH, Park K, J. Microbiol. Biotechnol., 25, 1108 (2015)

[16] Ma WC, Cao WJ, Zhang H, Chen KQ, Li Y, Ouyang PK, Biotechnol. Lett., 37(4), 799 (2015)

[17] Bhatia SK, Kim YH, Kim HJ, Seo HM, Kim JH, Song HS, Sathiyanar-ayanan G, Park SH, Park K, Yang YH, Bioprocess. Biosyst. Eng., 38, 2315 (2015)

[18] Friedl A, Qureshi N, Maddox IS, Biotechnol. Bioeng., 38, 518 (1991)

[19] Nakasaki K, Murai T, Akiyama T, Biotechnol. Bioeng., 33, 1317 (1989)

[20] Duarte JC, Rodrigues JA, Moran PJ, Valenca GP, Nunhez JR, AMB Express, 3, 31 (2013)

[21] Najafpour G, Younesi H, Ismail KSK, Bioresour. Technol., 92(3), 251 (2004)

[22] Barros MR, Cabral JM, Novais JM, Biotechnol. Bioeng., 29, 1097 (1987)

[23] Park MC, Lim JS, Kim JC, Park SW, Kim SW, Biotechnol. Lett., 27, 127 (2005)

[24] Lorenzoni ASG, Aydos LF, Klein MP, Ayub MAZ, Rodrigues RC, Hertz PF, J. Mol. Catal. B-Enzym., 111, 51 (2015)

[25] Agulhon P, Markova V, Robitzer M, Quignard F, Mineva T, Biomacromolecules, 13(6), 1899 (2012)

[26] Morch YA, Donati I, Strand BL, Skjak-Braek G, Biomacromolecules, 7(5), 1471 (2006)

[27] Mahajan R, Gupta VK, Sharma J, Indian J. Pharm. Sci., 72, 223 (2010)

[28] Bajpai SK, Sharma S, React. Funct. Polym., 59(2), 129 (2004)

[29] Kim YH, Kim HJ, Shin JH, Bhatia SK, Seo HM, Kim YG, Lee YK, Yang YH, Park K, J. Mol. Catal. B-Enzym., 115, 151 (2015)