In this paper, continuous observer is designed for a class of multi-output nonlinear systems with multi rate sampled and delayed output measurements. The time delay may be larger or less than the sampling intervals. The sampled and delayed measurements are used to update the observer whenever they are available. Sufficient conditions are presented to ensure global exponential stability of the observation errors by constructing a Lyapunov-Krasovskii function. A numerical example is given to illustrate the effectiveness of the proposed methods. (C) 2016 Elsevier Ltd. All rights reserved.