MicroRNAs (miRNAs) regulate gene expression by forming RNA-induced silencing complexes (RISCs). miRNA is an essential component of RISC for modulation of gene expression. Therefore, the release of miRNA from RISC is considered as effective method for inhibition of RISC activity. In this study, the releasing profiles of miRNA from RISC, using anti-miRNA oligonucleotides, are revealed.