Common findings of bla (CTX-M-55)-encoding 104-139 kbp plasmids harbored by extended-spectrum beta-lactamase-producing Escherichia coli in pork meat, wholesale market workers, and patients with urinary tract infection in Vietnam

Hoang TAV; Nguyen TNH; Ueda S; Le QP; Tran TTN; Nguyen TND; Dao TVK; Tran MT; Le TTT; Le TL; Nakayama T; Hirai I; Do TH; Vien QM; Yamamoto Y

Current Microbiology, Vol.74, No.2, 203-211, 2017

DOI10.1007/s00284-016-1174-x Export Citation

Common findings of bla (CTX-M-55)-encoding 104-139 kbp plasmids harbored by extended-spectrum beta-lactamase-producing Escherichia coli in pork meat, wholesale market workers, and patients with urinary tract infection in Vietnam

Hoang TAV, Nguyen TNH, Ueda S, Le QP, Tran TTN, Nguyen TND, Dao TVK, Tran MT, Le TTT, Le TL, Nakayama T, Hirai I, Do TH, Vien QM, Yamamoto Y

Extended-spectrum, beta-lactamase-producing Escherichia coli (ESBL-E) harboring the bla (CTX-M-55)-encoding plasmid (ESBL-E55) has been reported to be associated with urinary tract infection (UTI). The aims of this study were to clarify the prevalence of ESBL-E55 in pork meats and workers from the same wholesale market, as well as patients with UTI from a nearby hospital in Vietnam; we also investigated the plasmids encoding bla (CTX-M-55). Sequencing analysis showed that 66.6% of the ESBL-E isolated from pork meats contained bla (CTX-M-55), whereas the gene was present in 25.0% of workers and 12.5% of patients with UTI. Plasmid analysis showed that several sizes of plasmid encoded bla (CTX-M-55) in ESBL-E55 isolated from pork meats, whereas ESBL-E55 isolated from workers and patients with UTI contained only 104-139 kbp of bla (CTX-M-55)-encoding plasmids. This indicates that the 104-139 kbp sizes of bla (CTX-M-55)-encoding plasmids were commonly disseminated in pork meats, wholesale market workers, and patients with UTI.