Halotolerant microorganism was domesticated and used for the treatment of hypersaline organic wastewater containing acetone and dichloroethane. The degradation performance and the microbial population changes of the activated sludge were investigated. The degradation performance of the domesticated activated sludge for acetone and dichloroethane (94.3% and 88.4%) was much better than the un-domesticated activated sludge (17.8% and 74.1%). The main strains in domesticated sludge were salt-tolerant strains for organics degradation, indicating that the microbial community has been effectively domesticated and cultured.