In this work, the inverse analysis is applied to radiative heat transfer boundary design problems with non-gray media. The objective of the inverse problem is to find the power of the heaters on the heater surface that produces the desired output, that is, temperature and heat flux distribution over the design surface. The inverse problem is formulated as an optimization problem for minimization of an objective function, which is defined by the sum of the squared difference between estimated and desired heat flux distributions over the design surface. The non-gray optimization problem is solved using the conjugate gradient method, which is a gradient-based optimization method. The spectral line weighted-sum-of-gray-gases model (SLW) is used to account for non-gray gas radiation properties. The radiative transfer equation is solved by the discrete ordinates method combined with two models for simulation of non-gray media. Enclosures with diffuse and gray walls are considered. Radiation is assumed the dominant mode of heat transfer. Example problems including homogeneous/nonhomogeneous, isothermal/nonisothermal media are considered. The results obtained using the SLW model and the gray model are compared.