The impact of the beta-cyclodextrin (beta-CD) on the graphene oxide (GO) was considerably altered the activity of electrochemical sensors. Hence, the present study, we scrutinized the electrocatalytic determination of nitrobenzene (NB) by changing the different loading level of beta-CD on GO modified electrodes. The composites were prepared by the simple ultrasonication method and characterized by UV-Visible spectroscopy, infrared spectroscopy and scanning electron microscope. Interestingly, the synergistic electrocatalytic activity was appeared for the 1.2 mg beta-CD loaded GO (beta-CD1.2mg/GO) to the determination of NB whereas bare SPCE, GO and other beta-CD loaded GO/SPCE exhibited the lower electrocatalytic activity. The beta-CD1.2mg/GO composite modified SPCE was furnished the linear concentration range from 0.5-1000 mu M and showed the lowest detection limit of 0.184 mu M. Moreover, it exhibited high sensitivity, acceptable reproducibility and good stability. Besides, the proposed sensor was demonstrated its practicability in real water samples. (C) 2016 Elsevier Inc. All rights reserved.