In this contribution, a new carbon fiber microelectrode (CFME) for detection of hydrogen peroxide (H2O2) is constructed with a rapid and low-cost method based on the electrochemical etching technique and the electro-deposition of platinum (Pt) nanoparticles, and the potential application in electrochemical bioanalysis has been explored. It is observed that when the CFME modified in 5 mmol/L chloroplatinic acid with 0.5 mol/L H2SO4 used as supporting electrolyte, the as-prepared Pt modified carbon fiber microelectrode (Pt/CFME) demonstrates an excellent electrochemically catalytic activity, which exhibited a wide linear in relevant detection for the reduction of H2O2, ranging from 0.044 to 12.30 mmol/L with the limit of 44 mu mol/L. Moreover, our results illustrate that the Pt/CFME electrode can be readily utilized in scanning electrochemical microscope (SECM) studies to detect H2O2 release from human glioblastoma cells U87 when stimulated with ascorbic acid (AA). (C) 2016 Elsevier B.V. All rights reserved.