The objective of this article was to provide the extraction yields, the kinetic parameters, and the fatty acids compositions in the oil obtained from freeze-dried cells of Mornerellaisabellina. The oil was obtained by supercritical CO2 (SFE-CO2) and compressed liquefied petroleum gas (LPG) extractions in different conditions of temperature and pressure. According to the kinetic curves, the mass transfer rates, and the yields of extract, 80 degrees C/250 bar (SFE-CO2) and 40 degrees C/20 bar (LPG) were the most favorable conditions. The highest yield was 4.45 wt.% (LPG). According to the fatty acids composition, SFE-CO2 provided a slightly higher performance than compressed LPG. The highest concentration of fatty acids was 361 mg/g oil at 40 C/150 bar. Stearic, cis-10-pentadecanoic, cis-10-heptadecanoic, oleic, linoleic and linolenic acids were the major fatty acids extracted. These results highlight the possibility of using pressurized fluids to break the cells for increasing the fatty acids extraction from a lipids-rich fungal biomass. (C) 2016 Elsevier B.V. All rights reserved.