The cost-effective technique has been performed on 2-aminoethanethiol (2-AET) functionalized GQDs with mono-metallic and bi-metallic nanoparticles such as rod gold nanoparticles (rAuNPs) and rod gold-platinum nanoparticles (rAu-PtNPs). In present study, the structures of nanomaterials were highligted by transmission electron microscope (TEM), scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and X-ray diffraction (XRD) method. In addition, glassy carbon electrode (GCE) modified with rAu-PtNPs/GQDs nanomaterial (rAu-PtNPs/GQDs/GCE) was utilized in milk sample analysis. (C) 2016 The Electrochemical Society. All rights reserved.