A freeze drying technique was used to form a porous three-dimensional silk fibroin/collagen/hydroxyapatite sponge. Structure of sponge was analyzed using IR spectroscopy. Mechanical properties of sponge were tested and compared with composites containing different ratios of components in samples. Microstructure of porous 3D scaffolds was analyzed by Scanning Electron Microscope. Results showed that scaffolds made of blend of silk fibroin and collagen with inorganic particles of nanohydroxyapatite were less flexible and more stiff than biopolymeric sponge without inorganic part. Moreover, microstructure of those samples was less regular and the size of pores was bigger.