The saponins were extd. with MeOH sep. from flesh of fresh, heated (100 degrees C, 30 min) and stored (4 degrees C, 6 months) Bonel, Chrobry, Nochowski, Noe 21 and Rywal beetroot cultivars. The saponin content in the exts. depended on cultivar of beetroot and method for its processing. The highest saponin content was found in the exts. form pulp fresh Chrobry beetroot cultivar, and the smallest for Rywal cultivar. Furthermore, the heating and storage resulted in a decrease of the saponin content.