Yeast production and biomass biorefinery processes for lipid and carotenoid extraction generate residues that can be used as substrates for anaerobic digestion. Glucose and carob pulp syrups were used as carbon sources to produce the yeast biomass. The yeast cultivation broth, yeast biomass residues (after carotenoid and lipid extraction) and the carob pulp solid residues obtained from the extraction of sugars were used to produce biogas by applying different Substrate/Inoculum ratios (S/I of 0.5 and 0.75). For all the residues studied, the digestions at the S/I ratio of 0.75 provided higher biogas yields than those carried out at the S/I ratio of 0.5. The best results in terms of biogas production and methane yield were observed for the yeast residue digestion at S/I of 0.75 (65.9 mL, 333.7 mL g(-1) VS-1 substrate). As monitored through flow cytometry, its bacterial consortium showed the lowest proportion of injured cells. (C) 2016 Elsevier Ltd. All rights reserved.