The kinetics of sucrose hydrolysis by invertase was studied in order to find a comprehensive model for the reaction pathway and mechanism. First, three common models of Michaelis-Menten (MM), substrate inhibition (S-2), and substrate clusters' inhibition (S-3(I)) were investigated. The third model was found to better predict the initial sucrose concentration. Then, the S-3(I) model was modified to cover the remaining pathway (S-3(II)). Finally, a new comprehensive model (S-3(III)) was evaluated, which in addition to what is considered in the two previously mentioned models (S-3(I) and S-3(II)) also involved the initial time lag. The model predictions showed an excellent agreement with the experimental data. The mean absolute error for the MM model is significantly reduced for the S-3(III) model.