Electrochemical biosensors are responsible for quantification of analytes for medical diagnostics applications. They are considered as a promising means to investigate the content of a biological sample owing to the direct exchange of a biological process to an electronic output signal. Novel characteristics of nanocarbon materials attracted much attention for fabrication of numerous electrochemical biosensors with developed analytical capacities. This paper aims to provide perceptions of 3D graphene-based electrochemical biosensors and to demonstrate its application in glucose detection. The developed glucose biosensing platform exhibits excellent catalytic activity towards glucose detection over a wide linear range of up to 6 mM with sensitivity of 1.63 mu A mM(-1) cm(-2) and the stability of electrode is around 76.9% after one month. The facile and easy electrochemical approach used for the preparation of 3DG-GOD modified GCE may open up new horizons in the production of cost-effective biosensors. (C) 2016 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.