Despite cyanoacrylate's numerous advantages such as good cosmetic results and fast application for first aid, drawbacks such as brittleness and local tissue toxicity have limited their application. In this study, to improve both the biocompatibility and mechanical properties of cyanoacrylate, allyl 2-cyanocarylate was pre-polymerized and mixed with poly(L-lactide-co-epsilon-caprolactone) (PLCL, 50: 50) as biodegradable elastomer and mixed with hydroxyapatite (HA) as multifunctional bio-glue for variety body application. Hybridized allyl 2-cyanoacrylate (Hybridized Cy) was mixed with pre-polymerization allyl 2-cynaocarylate and PLCL. For the evaluation of various properties of hybridized Cy/HA bio-glue mixtures, bond strength, surface analysis (SEM, XRD), cell viability, and biocompatibility were confirmed. Especially, optimal condition for hybridized Cy/HA bio-glue was determined as 1.2 wt% HA mixture ratio through bond strength. Surface morphology has smooth form to increase HA mixture ratio. Cell viability increased with optimal ration of hybridized Cy/HA. These results indicate that hybridized Cy/HA bio-glue can be used widely as advanced adhesive in various fields.