AimsThe aim of this study was to detect and quantify Aichi virus (AiV) in shellfish from three estuaries in Galicia, the main producer of molluscs in Europe. Methods and ResultsA total of 249 shellfish samples were analysed using a reverse transcription-quantitative PCR procedure. AiV was detected in 15 of 249 (602%) samples. Ria de Ares-Betanzos showed the highest prevalence (111%), followed by Ria do Burgo (37%) and Ria de Vigo, (256%). AiV quantifications ranged from nonquantifiable (under the limit of quantification of the method) to 69x10(3) RNAc per g DT, with a mean value of 19x10(2) RNAc per g DT. ConclusionResults obtained indicated that the prevalence of this enteric virus in the studied area is considerably lower than those of other enteric viruses, such as Norovirus, Sapovirus, HAV or HEV. Significance and Impact of the StudyThis is the first study that detects the presence of AiV in shellfish from authorized harvesting areas in Spain. Further studies with clinical samples are needed to determine the potential risk of AiV for human health in Galicia.