The present study deals with the separation of lactic acid (LA) by a polymeric adsorbent Amberlite XAD-7. Equilibrium and kinetic studies were carried out to investigate adsorption of LA on Amberlite XAD-7 at three different temperatures (298, 308, and 318 K). Equilibrium studies revealed the optimum dose of Amberlite XAD-7 as 2.5 g. The adsorption equilibrium time was determined as 90 min. Adsorption isotherms such as Freundlich, Langmuir, Temkin, and Dubinin-Radushkevich were validated using the obtained experimental data. Langmuir isotherms showed better fitting with the experimental data with R-2 = 0.99. Pseudo-second-order model and Elovich model were used to fit the adsorption system. Study also revealed 99% removal of LA using Amberlite XAD-7.