In this paper, a high-performance three dimensional (3D) bio-platform of glucose oxidase (GOD) adsorbed on Au nanoparticles (AuNPs) assembled polyaniline (PANi) nanowires network (GOD/AuNPs/PANi) was constructed to sensitively detect glucose by electrochemiluminescent (ECL) technology for the first time. Due to the unique structure of porous network, excellent conductivity and good biocompatibility for enzyme, the bio-platform enable highly strong the ECL emission, which make the proposed biosensor achieves a wide linear range of 0.1-100 mu M (R-2 = 0.992), extremely low detection limit of 0.05 mu M (S/N = 3), excellent selectivity and good stability in glucose detection. When the proposed biosensor was used in serum samples determination, a satisfactory result also obtained. (C) 2017 Elsevier B.V. All rights reserved.