Antifouling paint particles (APPs) are generated during periodical maintenance of boat hulls. Chemical composition and toxicity (either chronic or acute) of APPs found in the sediment was evaluated using the epibenthic copepod Nitokra sp. The APPs analyzed showed the presence of high levels of metals such as Cu (234,247 +/- 268 mu gg(-1)), Zn (112,404 +/- 845 mu gg(-1)) and the booster biocide DCOIT (0.13 mu gg(-1)). Even at low concentrations (as from 5 mgg-1 of APPs by mass of sediment) a significantly decrease in the fecundity was observed in laboratory tests. When the sediment was disturbed in elutriate test, a LC50 of 0.14% for APPs was found. This study was the first assessment of toxicity associated with the presence of APPs in sediment to benthic organisms, and it calls attention to the need of improving regulations in boatyards and marina areas. (C) 2017 Elsevier B.V. All rights reserved.