In this work, we present a mild one-step enzymatic-catalyzed copolymerization of diethyl succinate, 1,4-butanediol and p-dioxanone (PDO) to synthesize poly(p-dioxanone-co-butylene-co-succinate) copolyesters. Their chemical structure, triad distributions, and molecular weight of the copolyesters are characterized by H-1 NMR, C-13 NMR and GPC. All copolyesters have narrow molecular weight polydispersity (1.3-1.5) and higher molecular weight (M-w) values (23700-33200 g mol(-1)), which are well controlled by varying the amount of PDO units from 9 to 30 mol%. NMR, DSC, and XRD results show that copolyesters are semicrystalline random copolyesters and thermal properties depended on their composition. Furthermore, the degradation rate of the poly(p-dioxanone-co-butylene-co-succinate) are enhanced compared to PBS. (C) 2017 Elsevier Ltd. All rights reserved.