방사선 이용 상처치료용 β-Glucan 하이드로젤 제조 및 특성 분석

권희정; 안성준; 정진오; 김수민; 박종석; 정성린; 노영창; 임윤묵; Hui-Jeong Gwon; Sung-Jun Ahn; Jin-Oh Jeong; Su-Min Kim; Jong-Seok Park; Sung In Jeong; Young-Chang Nho; Youn-Mook Lim

Polymer(Korea), Vol.41, No.4, 586-591, July, 2017

DOI10.7317/pk.2017.41.4.586 Export Citation

방사선 이용 상처치료용 β-Glucan 하이드로젤 제조 및 특성 분석

Preparation and Characterization of Radiation Fabricated β-Glucan Hydrogels for Wound Dressing

권희정¹, 안성준¹, 정진오^{1, 2}, 김수민^{1, 3}, 박종석¹, 정성린¹, 노영창¹, 임윤묵^{1, †}

¹한국원자력연구원 첨단방사선연구소 공업환경연구부
²광주과학기술원 신소재공학부
³한양대학교 생명공학과

Hui-Jeong Gwon¹, Sung-Jun Ahn¹, Jin-Oh Jeong^{1, 2}, Su-Min Kim^{1, 3}, Jong-Seok Park¹, Sung In Jeong¹, Young-Chang Nho¹, and Youn-Mook Lim^{1, †}

¹Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 1266 Sinjeong-dong, Jeongeup-si, Jeollabuk-do 56212, Korea
²School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Korea
³Department of Bioengineering, Division of Applied Chemical and Bio Engineering, Hanyang University, Seoul 04763, Korea

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초록

화학가교제의 사용없이 감마선을 이용하여 β-glucan, poly(vinyl alcohol)(PVA), poly(vinyl pyrrolidone)(PVP),κ-carrageenan(κC), glycerin으로 구성된 고분자 하이드로젤을 제조하였다. 25, 50, 75 kGy로 감마선 조사선량을 달리하여 하이드로젤을 제조한 후, 젤화율, 팽윤도, 압축강도의 측정을 통해 제조된 하이드로젤의 물리적 특성을 확인 하였다. 조사선량의 증가에 따라 하이드로젤의 가교밀도가 증가되어 젤화율과 압축강도는 증가하였고, 팽윤도는 감소되었다. 제조된 베타글루칸 하이드로젤로 손상된 조직의 재생 특성을 평가해 보기 위해 동물실험을 수행하였고, 그 결과 베타글루칸이 함유된 하이드로젤이 빠른 상처 치유능을 나타내었고, 상용제품과 비교 시에도 동등한 치료효과를 관찰할 수 있었다. 따라서 감마선으로 제조된 β-glucan/PVA/PVP/κC/glycerin 하이드로젤은 화학가교제 독성의 우려 없이 β-glucan에 의한 향상된 치료효과와 더불어 향후 조직재생용 소재로서 유용하게 사용될 수 있을 것으로 사료된다.

Hydrogels consisted of β-glucan, poly(vinyl alcohol) (PVA), poly(vinyl pyrrolidone) (PVP), κ-carrageenan (κC) and glycerin were prepared by gamma-ray irradiation for damaged tissue regeneration. Irradiation doses of 25, 50, and 75 kGy were exposed, respectively, to the β-glucan hydrogel to evaluate the effect of irradiation dose on physical properties. The physical properties were examined such as gel fraction, absorption ratio, and compressive strength. It was found that the gel fraction and the compressive strength increased with increasing the irradiation dose. This is due to the fact that the crosslinking density increases with increasing the irradiation dose, whereas the absorption ratio decreased with increasing the irradiation dose. On observing the wound healing of rat skin, the resulting hydrogels accelerated the wound repair, which can be attributed to the release of β-glucan from the hydrogel. Therefore, radiation fabricated β-glucan/ PVA/PVP/κC/glycerin blended hydrogel was suitable for wound healing and could be considered as good tissue regeneration biomaterials without chemical toxicity.

Keywords:gamma-ray;irradiation;hydrogels;tissue regeneration;wound dressing

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