화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.488, No.1, 165-170, 2017
Delta 12-fatty acid desaturase is involved in growth at low temperature in yeast Yarrowia lipolytica
We investigated the role of FAD2, which was predicted to encode a fatty acid desaturase of the n-alkane-assimilating yeast Yarrowia lipolytica. Northern blot analysis suggested that FAD2 transcription was upregulated at low temperature or in the presence of n-alkanes or oleic acid. The FAD2 deletion mutant grew as well as the wild-type strain on glucose, n-alkanes, or oleic acid at 30 degrees C, but grew at a slower rate at 12 degrees C, when compared to the wild-type strain. The growth of the FAD2 deletion mutant at 12 degrees C was restored by the addition of 18:2, but not 18:1, fatty acids. The amount of 18:2 fatty acid in the wild-type strain was increased by the incubation at 12 degrees C and in the presence of n-octadecane. In contrast, 18:2 fatty acid was not detected in the deletion mutant of FAD2, confirming that FAD2 encodes the Delta 12-fatty acid desaturase. These results suggest that Delta 12-fatty acid desaturase is involved in the growth of Y. lipolytica at low temperature. (C) 2017 Elsevier Inc. All rights reserved.