This study reports production of microbial oil from a yeast strain Trichosporon sp., (RW) isolated from decayed wood. Preliminary analysis based on fluorescence microscopy and spectroscopy of Nile red stained yeast cells showed accumulation of lipid globules. The potential of the yeast to produce lipids was evaluated on glucose, glycerol and acid hydrolysate of sugarcane bagasse, where Trichosporon sp. (RW) was found to accumulate 21.45 (59.6%), 18.41 (56%) and 10.25 g/l (40.5%) of the lipids after 120 h of fermentation at 30 degrees C. FAME analysis of lipids by GC-FID and NMR revealed oleic acid (18: 1) as the major constituent, corresponding to 50.05, 46.48 and 54.66% of the accumulated lipids in glucose, glycerol and hydrolysate grown cultures, respectively. Other accumulated lipids included palmitic (16: 0), linoleic (18: 2) and stearic acids (18: 0) in that order. The cetane number of the lipids ranged from 52.39 to 59.57 indicating suitability for biodiesel production. (C) 2017 Elsevier Ltd. All rights reserved.