Three alternatives for bioethanol production from pretreated mango stem bark after maceration (MSBAM) were evaluated as a biorefinery component for the mango agroindustry. These included separate hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF), and pre-saccharification followed by simultaneous saccharification and fermentation (PSSF). The effects on ethanol concentration, yield and productivity of pretreated MSBAM solids loading, Tween 20 addition, and temperature were used for process comparisons. The highest yields for the SHF, SSF, and PSSF process alternatives were 58.8, 81.6, and 84.5%, respectively. Since saccharification and fermentation are carried out in the same vessel in the SSF alternative, and no significant SSF and PSSF differences in ethanol concentration were observed, SSF is recommended as the best process configuration. (C) 2017 Elsevier Ltd. All rights reserved.