Removal of nutrients (phosphorus and nitrogen) as struvite from bio-oil aqueous phase generated via hydrothermal liquefaction of algae was evaluated in this study. Effect of process parameters such as pH, temperature and reaction time on struvite formation was studied. More than 99% of phosphorus and 40-100% ammonium nitrogen were removed under all experimental conditions. X-ray diffraction analysis confirmed the formation of struvite, and the struvite recovered from bio-oil aqueous phase can be used as a slow-release fertilizer. Biogas production from struvite recovered bio-oil aqueous phase showed 3.5 times higher CH4 yield (182 +/- 39 mL/g COD) as compared to non-struvite recovered aqueous phase. The results from this study indicate that both struvite and methane can be produced from bio-oil aqueous phase. (C) 2017 Elsevier Ltd. All rights reserved.