In the present work, sensitivity analyses are performed on a plant-wide model incorporating the typical treatment unit of a full-scale wastewater treatment plant and N2O production and emission dynamics. The influence of operating temperature is investigated. The results are exploited to identify the biological mechanisms responsible for N2O emissions, TN removal efficiency, competition for oxygen among the different microbial groups and the trade-off between oxygen consumption and effluent nitrogen loading. It was found that N2O emissions are triggered by poor oxygenation levels which cause an imbalance in the activity of NOB over the activity of AOB. As a matter of fact this imbalance leads to nitrite accumulation which in turn triggers AOB denitrification. This is particularly true at high temperatures, due to higher difference between AOB and NOB specific growth rates. At the same time, too high oxygen availability is found to inhibit heterotrophic denitrification, leading to incomplete reduction of nitrogen oxides and thereby to an accumulation of nitrous oxide. High oxygen supply is also found to worsen effluent quality via inhibition of heterotrophic denitrification. Low temperatures have shown to drastically limit aerobic AOB activity, thus compromising effluent quality. Finally, the organic biodegradable carbon surplus leaving the anoxic zone is identified to slow down NOB activity via oxygen competition with heterotrophs in the aerobic zone. With regard to the control strategy for the minimization of N2O emissions, the ratio between nitrate produced and ammonium consumed in an aerobic zone should be considered as candidate controlled variable to check whether nitrification is complete or nitrites are building up. Oxygen availability should be regulated according to the measured controlled variable. (C) 2017 Elsevier B.V. All rights reserved.