The objective of this study is to examine the segregation and interdiffusion-induced migration effects in the course of interaction of particles differing in size, density and roughness while undergoing rapid gravity-driven granular flows on a vibrated rough chute. An experimental and analytical method is proposed in order to obtain the profiles of velocity, fraction of the void volume and test particle distribution along the bed depth. It was found out that interdiffusion migration is the main physical mechanism of nonuniform particle interaction determining separation effect in rapid gravity-driven granular flows of granular materials on a rough chute vibrating at low frequencies. Segregation dominates when high frequencies of vibrating chute take place. (C) 2017 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.