Dairy factories manufacturing Greek yoghurt generate huge quantities of acid whey, an effluent which has serious ecological ramifications. In this work, we have used Actinopolyspora halophila (MTCC 263), an extreme halophile that utilized acid whey for pilot scale production of glycine betaine and trehalose which are commercially important compounds having innumerable applications in many industries. Batch bioreactor process with 200 rpm agitation and 3 win aeration gave a maximum production of 13.57 +/- 0.39 g L-1 glycine betaine and 5.09 +/- 0.19 g L-1 of trehalose. Kinetic models were also evaluated for production (biomass, glycine betaine and trehalose) and substrate utilization (lactose, glycine and total nitrogen) in A. halophila. Screening of resins for chromatographic separation of glycine betaine and trehalose showed strong cation exchange resin to be the most effective. The recovery of glycine betaine was 86.04 +/- 2.86% with purity of 82.26% and that of trehalose was 90.04 +/- 1.17% with purity of 94.47%. The findings of this work could benefit the dairy industries to plan on utilization of acid whey and generate revenue while simultaneously preventing environmental and economic repercussions caused by it. (C) 2017 Elsevier Ltd. All rights reserved.