Chemical Physics Letters, Vol.683, 543-546, 2017
Broadband stimulated Raman spectroscopy in the deep ultraviolet region
We report broadband stimulated Raman measurements in the deep ultraviolet (DUV) region, which enables selective probing of the aromatic amino acid residues inside proteins through the resonance enhancement. We combine the narrowband DUV Raman pump pulse (< 10 cm(-1)) at wavelengths as short as 240 nm and the broadband DUV probe pulse (>1000 cm(-1)) to realize stimulated Raman measurements covering a > 1500 cm(-1) spectral window. The stimulated Raman measurements for neat solvents, tryptophan, tyrosine, and glucose oxidase are performed using 240- and 290-nm Raman pump, highlighting the high potential of the DUV stimulated Raman probe for femtosecond time-resolved study of proteins. (C) 2017 Elsevier B.V. All rights reserved.