Mixed vesicles with complementary moieties were prepared by cosonication of didodedcyidimethylammonium bromide with 5,5-didodecylbarbituric acid and/or 9-hexadecyladenine. These vesicles, bearing recognizable moieties at the interface, were more stable than the simple vesicles prepared from didodecyldimethylammonium bromide. Phase contrast optical and AFM microscopies were used for imaging these vesicles and also for revealing that the interaction occurring between the recognizable moieties of mixed vesicles leads to the formation of larger aggregates.