라벤더(Lavandula angustifolia) 추출물 및 분획물의 세포보호효과와 활성 성분 분석

김아영; 하지훈; 김아랑; 정효진; 김경미; 박수남; A Young Kim; Ji Hoon Ha; A Rang Kim; Hyo Jin Jeong; Kyoung Mi Kim; Soo Nam Park

Applied Chemistry for Engineering, Vol.28, No.4, 479-484, August, 2017

DOI10.14478/ace.2017.1051 Export Citation

라벤더(Lavandula angustifolia) 추출물 및 분획물의 세포보호효과와 활성 성분 분석

Cellular Protective Effect and Active Component Analysis of Lavender (Lavandula angustifolia) Extracts and Fractions

김아영 , 하지훈 , 김아랑 , 정효진 , 김경미 , 박수남 ^†

서울과학기술대학교 정밀화학과, 화장품종합기술연구소

A Young Kim , Ji Hoon Ha , A Rang Kim , Hyo Jin Jeong , Kyoung Mi Kim , and Soo Nam Park^†

Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology, 232 Gongneungro, Nowon-gu, Seoul 01811, Korea

E-mail:

초록

본 연구에서는 라벤더 70% 에탄올 추출물 및 분획물의 항산화 활성과 세포보호효과를 조사하였다. 자유라디칼 (1,1-phenyl-2-picrylhydrazyl, DPPH) 소거 활성(FSC50)은 라벤더 추출물, 에틸아세테이트 및 아글리콘 분획에서 각각 46.6, 45.5 및 477.5 μg/mL를 나타냈다. Fe3+-EDTA/H2O2계에서의 총항산화능(OSC50)은 라벤더 추출물, 에틸아세테이트 및 아글리콘분획에서 각각 8.1, 3.3 및 17.6 μg/mL이었으며, L-ascorbic acid의 OSC50 (1.5 μg/mL)보다 낮은 활성을 나타냈다. 1O2으로 유도된 적혈구 광용혈 실험에서 아글리콘 분획은 매우 큰 세포보호활성을 나타냈다. 50 μM 농도에서 라벤더 추출물, 에틸아세테이트 및 아글리콘 분획의 세포보호효과(τ50)가 70.6, 87.2 및 165.2 min으로 나타났다. 특히, 라벤더 아글리콘 분획은(+)-α-tocopherol 보다 3.8배 높은 세포보호효과를 나타냈다. TLC 및 LC-MS를 이용하여 라벤더 분획의 성분(luteolin 7-O-glucuronide, vitextin, rosmarinic acid, luteolin, apigenin)을 확인하였으며, 아글리콘 분획은 에틸아세테이트 분획에 비해 주요 플라보노이드(luteolin, apigenin)는 크게 증가하지 않았다. 결론적으로 라벤더가 화 장품의 항산화 소재로써 응용 가능함을 시사한다.

In this study, antioxidative activities and cellular protective effects of 70% ethanol extracts and fractions from lavender were evaluated. The scavenging activity (FSC50) of free radical (1,1-phenyl-2-picrylhydrazyl, DPPH) was 46.6, 45.5 and 477.5 μg/mL in the 70% ethanol extract, ethyl acetate fraction and aglycone fraction, respectively. The reactive oxygen species scavenging activities (OSC50) of 70% ethanol extract, ethyl acetate fraction and aglycone fraction were 8.1, 3.3 and 17.6 μg/mL, respectively, and they showed lower antioxidative activity than that of using L-ascorbic acid (1.5 μg/mL). However, the aglycone fraction showed higher photohemolysis protective effect than that of using the 70% ethanol extract and ethyl acetate fraction. At 50 μM concentration, the cellular protective effect (τ50) of 70% ethanol extract, ethyl acetate fraction and aglycone fraction from lavender was 70.6, 87.2 and 165.2 min, respectively. In particular, the lavender aglycone fraction showed 3.8 times higher cellular protective effect than that of (+)-α-tocopherol. The lavender fractional components including luteolin 7-O-glucuronide, vitextin, rosmarinic acid, luteolin, and apigenin were identified using TLC and LC-MS. However, the lavender aglycone fraction did not show any significant increase in flavonoids (luteolin and apigenin) compared to that of the ethyl acetate fraction. In conclusion, it is suggested that lavender may be applied as an antioxidant material in cosmetic industries.

Keywords:Lavandula angustifolia;antioxidant activity;component;natural material

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Yaar M, Gilchrest BA, Br. J. Dermatol., 157(5), 874 (2007)
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Kahl R, Kappus H, Z. Lebensm. Unters. Forsch., 196, 329 (1993)
Magdalena LC, Tak YA, Free Radic. Biol. Med., 48(6), 749 (2010)
Spiridon I, Colceru S, Anghel N, Teaca CA, Bodirlau R, Armatu A, Nat. Prod. Res., 25(17), 1657 (2011)
Ahn YJ, Won BR, Kang MK, Kim JH, Park SN, J. Soc. Cosmet. Sci. Korea, 35(2), 125 (2009)
Dudaa SC, Marghitas LAI, Dezmireana D, Dudab M, Margaoana R, Bobis O, Ind. Crops Prod., 77, 499 (2015)
Evandri MG, Battinelli L, Daniele C, Mastrangelo S, Bolle P, Mazzanti G, Food Chem. Toxicol., 43(9), 1381 (2005)
Hajhashemi V, Ghannadi A, Sharif B, J. Ethnopharmacol., 89(1), 67 (2003)
Baptista R, Madureira A, Jorge R, Ado R, Duarte A, Duarte N, Lopes MM, Teixeira G, Evid. Based Complement. Alternat. Med., 2015, 1 (2015)

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[1] Afonso V, Champy R, Mitrovic D, Collin PI, Lomri A, Joint Bone Spine, 74(4), 324 (2007)

[2] Yaar M, Gilchrest BA, Br. J. Dermatol., 157(5), 874 (2007)

[3] Park SN, Effects of Flavonoids and Other Phenolic Compounds on Reactive Oxygen Mediated Biochemical Reactions, PhD Dissertation, Seoul National University, Seoul, Korea (1989).

[4] Lee DS, Lim MS, Kwan SS, Kim SY, Park SN, Appl. Chem. Eng., 23(1), 93 (2012)

[5] Davies MJ, Am. Heart J., 23, 2382 (1998)

[6] Bagchi D, Bagchi M, Hassoun EA, Stohs SJ, Toxicology, 104(1-3), 129 (1995)

[7] Berman SB, Hastings TG, J. Neurochem., 69(3), 1185 (1997)

[8] Park SN, J. Korean Ind. Eng. Chem., 14(5), 657 (2003)

[9] Yamakoshi J, Otsuka F, Sano A, Tokutake S, Saito M, Kikuchi M, Kubota Y, Pigment Cell Res., 16, 629 (2003)

[10] Park SN, Korean J. Food Sci. Technol., 35, 510 (2003)

[11] Kyrtopoulos SA, Cancer Surv, 8(2), 423 (1988)

[12] Sohala RS, Orrb WC, Free Radic. Biol. Med., 52(3), 539 (2012)

[13] Kahl R, Kappus H, Z. Lebensm. Unters. Forsch., 196, 329 (1993)

[14] Magdalena LC, Tak YA, Free Radic. Biol. Med., 48(6), 749 (2010)

[15] Spiridon I, Colceru S, Anghel N, Teaca CA, Bodirlau R, Armatu A, Nat. Prod. Res., 25(17), 1657 (2011)

[16] Ahn YJ, Won BR, Kang MK, Kim JH, Park SN, J. Soc. Cosmet. Sci. Korea, 35(2), 125 (2009)

[17] Dudaa SC, Marghitas LAI, Dezmireana D, Dudab M, Margaoana R, Bobis O, Ind. Crops Prod., 77, 499 (2015)

[18] Evandri MG, Battinelli L, Daniele C, Mastrangelo S, Bolle P, Mazzanti G, Food Chem. Toxicol., 43(9), 1381 (2005)

[19] Hajhashemi V, Ghannadi A, Sharif B, J. Ethnopharmacol., 89(1), 67 (2003)

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