AimThis study evaluated differences between two strains of Ceriporiopsis subvermispora on improving the nutritive value and invitro degradability of wheat straw. Methods and ResultsWheat straw was treated with the fungi for 7weeks. Weekly samples were analysed for ergosterol content, invitro gas production (IVGP), chemical composition and lignin-degrading enzyme activity. Ergosterol data showed CS1 to have a faster initial growth than CS2 and reaching a stationary phase after 3weeks. The IVGP of CS1-treated wheat straw exceeded the control earlier than CS2 (4 vs 5weeks). CS1 showed a significantly higher (P<0001) selectivity in lignin degradation compared to CS2. Both strains showed peak activity of laccase and manganese peroxidase (MnP) at week 1. CS1 showed a significantly higher (P<0001) laccase activity, but lower (P=0008) MnP activity compared to CS2. ConclusionBoth CS strains improved the nutritive value of wheat straw. Variation between strains was clearly demonstrated by their growth pattern and enzyme activities. Significance and Impact of the StudyThe differences among the two strains provide an opportunity for future selection and breeding programs in improving the extent and selectivity of lignin degradation in agricultural biomass.