Herein, we report the synthesis of Vanadium Pentoxide Nanoflakes (V2O5 NF) using ionic liquid and employed the V2O5 NF in electrochemical determination of Morin (MR) in fruit samples. The V2O5 NF were characterized by Powder X-ray diffraction (PXRD), scanning electron microscope (SEM), energy dispersive X-ray analyzer (EDX), differential pulse voltammetry (DPV), and cyclic voltammetry (CV). Remarkably, the as-synthesized V2O5 NF exhibited excellent electrochemical behavior and electrochemical ability towards MR. The CV and DPV studies were recognized that the electrochemical performance of V2O5 NF film modified glassy carbon electrode (V2O5 NE/GCE) towards detection of MR is outstanding in comparison with unmodified GCE. The proposed MR sensor shows a wide linear range, high sensitivity, and low limit of detection are 0.05-10.93 mu m, 1.130 mu A mu M-1 cm(-2), and 9 nM respectively. The fabulous analytical parameters of the developed sensor surpassed the previously reported modified electrodes, rendering the potential application of V2O5 NF in environmental, biomedical, and pharmaceutical samples. (C) 2017 Published by Elsevier Inc.