The conformational analysis of glycosidases affords a route to their specific inhibition through transition-state mimicry. Inspired by the rapid reaction rates of cyclophellitol anti cyclophellitol aziridine both covalent retaining beta-glucosidase inhibitors we postulated that the' corresponding carba "cyclopropyl" analogue would be a potent retaining beta-glucosidase inhibitor for those enzymes reacting through the H-4(3) transition-state conformation. Ab initio metadynamics simulations of the conformational free energy landscape for the cyclopropyl inhibitors show a strong bias for the H-4(3) conformation, and carba-cyclophellitol, with an N-(4-azidobutyl)carboxamide moiety, proved to be a potent inhibitor (K-i = 8.2 nM) of the Thermotoga maritima TmGH1 beta-glucosidase. 3-D structural analysis and comparison with unreacted epoxides show that this compound indeed binds in the H-4(3) conformation, suggesting that conformational strain induced through a cyclopropyl unit may add to the armory of tight-binding inhibitor designs.