Nitrogen-enriched nanobiopolymer has been fabricated using (2,3-epoxypropyl) trimethylammonium chloride (EPTMAC) and explored for the removal of Cr(VI) from tannery effluent. The removal efficiency of nanobiopolymer was found to be 23.99 mg g(-1) (95.94%) under optimized conditions. The sorption data agrees well with the Langmuir and pseudo-second-order model. Equilibrium parameter (R-L) and sorption energy show the favorability and physical binding of Cr(VI) on the nanobiopolymer's backbone. The values of G degrees (-7.84 kJ mol(-1)), S degrees (65.97 J mol(-1)K(-1)) and H degrees (11.82 kJ mol(-1)) reflect the feasible nature of the sorption process. Reusability study was also conducted to state the performance of the nanobiopolymer.