Antifouling paints have been used to prevent corrosion of the surface of the ship hulls (vessels) and the adhesion of marine organisms from these surfaces. And the development of a new type of antifouling paint resin which has excellent antifouling performance and no persistence in the marine ecology is necessary. In this study, we have studied polyurethane (PU) and waterborne polyurethane (WPU) resins which are considered to be applied as a new antifouling paint matrix. PU and WPU resins were synthesized by 4,4'-diphenylmethane diisocyanate (MDI), 2,2'-bis(hydroxymethyl) butyric acid (DMBA), 1,4-butanediol, polyethylene glycol (PEG) as ether type polyol and poly(tetramethylene adipate) glycol (PTAd) as ester type polyol. And then quaternary ammonium salts (QAS) which are effective biocides were substituted. After that, the synthesized PU and WPU resins were tested by the laser scan micrometer to confirm the self-polishing property with different types of polyol by measuring film thickness in sea water.