Nanometer-scale gold particles exhibit size-dependent electronic properties with important sensing and biosensing applications. In the same way, a lot of analytes show some type of surface-sensitive reaction and the electrode material has a strong influence on the catalytic activity. In this work we study the kinetics and electrochemistry of electrodes with size controlled gold nanoparticles, obtained by electrodeposited amidoferrocenylpoly(propyleneimine) dendrimers of two generations as templates, and the kinetics and the analytical response to the oxidation of dopamine. We demonstrate that the four-types of modified electrodes show good catalytic responses toward the oxidation of dopamine via different processes in relation with the absence or presence of gold nanoparticles and their size. The best response was obtained with the largest nanoparticles, obtained with the first generation dendrimer-template at 0.3 V vs. SCE, with three linear ranges (0-70, 70-600 and 600-1000 mu M), with sensitivities 585.7; 466.0 and 314.3 mu A/mM cm(2), and limit of detection of 0.01 mu M. The effect of interfering substances has been studied by differential pulse voltammetry and the developed sensor has been successfully used for the determination of dopamine in a commercial dopamine hydrochloride injection and in spiked Human urine. (C) 2017 Elsevier B.V. All rights reserved.