In this study, the expression of 4 genes encoding alpha-acetolactate synthase, a-acetolactate decarboxylase, 2,3-butanediol dehydrogenase, and NADH oxidase was modulated using a previously developed cocktail delta-integration strategy. The resultant strain, YPH499/dPdAdG/BD6-10, was used in a fed-batch cultivation for the production of 2,3-butanediol. The concentration, production rate, and yield obtained were 80.0 g/L, 4.00 g/L/h, and 41.7%, respectively. The production rate and yield of the compound obtained are higher for this strain compared to reports published for Saccharomyces cerevisiae so far. The cocktail d-integration strategy allows for modulation of multiple gene expression, without the exact knowledge of rate-limiting steps, and therefore, could be used as a promising strategy for the production of bio-based chemicals in recombinant S. cerevisiae. (C) 2017 Elsevier Ltd. All rights reserved.